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The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a 'green' technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis.
This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed.This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method. Key Features.
DedicationPrefaceList of Contributors1. Solid-Phase Microextraction in Perspective1.1. Sample Preparation as Part of the Analytical Process1.2. Classification of Extraction Techniques1.3. Perspective on Microextraction Techniques1.4.
However, there are no reports of headspace solid- phase microextraction–gas chromatography–mass spectrometry (HS–SPME–GC–MS) of volatile compounds in strawberries grown in soilless systems. This is a PDF file of an unedited manuscript that has been. Solid phase microextraction (SPME) 544 applied to honey quality.
Implementations of SPME1.5. Miniaturisation and Integration1.6. In Vivo Analysis1.7. SPME Versus SPE2. Theory of Solid-Phase Microextraction2.1. SPME Principle2.3. Extraction with Derivatisation2.6.
Extraction of Sample Matrices Containing Solids2.7. Solid Versus Liquid Sorbents2.8. Passive TWA Sampling2.9. In-Tube SPME2.10. Experimental Verification3.
Development of SPME Devices and Coatings3.1. Historical Perspective3.2. Rational Design of SPME Devices3.3. On-Site Samplers3.4. Development of New SPME Coatings3.5.
Interfaces to Analytical Instrumentation4. SPME Commercial Devices and Fibre Coatings4.1. Description of SPME Fibre Assemblies and Holders4.3. Description of Fibre Cores, Coatings and the Coating Process4.4. A Guide for the Selection of the Appropriate SPME Fibre5. Automated SPME Systems5.1. Automated Solid-Phase Microextraction–Gas Chromatography5.2.
Automated SPME–LC5.3. Other Automated Configurations Involving SPME6. Traditional Calibration Methods for the Quantification of SPME6.3. Equilibrium Extraction6.4. Exhaustive Extraction6.5. Diffusion-Based Calibration6.6.
Calibration of SPME by Liquid Injection6.7. Solid-Phase Microextraction Method Development7.1. SPME Method Development – General7.3. SPME Method Development for GC Applications7.4.
SPME Method Development for HPLC Applications7.5. Method Validation7.6. Concluding Remarks8. SPME and Environmental Analysis8.1.
Fibre SPME8.3. In-Tube SPME8.4. Applications of SPME in Various Environmental Sample Matrices8.6. Applications of SPME for Various Analytes in Environmental Samples8.7. Concluding Remarks9. Application of Solid-Phase Microextraction in Food and Fragrance Analysis9.1.
Introduction and Method Development Considerations9.2. Reviews and Case Studies Involving SPME as an Extraction Procedure9.3. Concluding Remarks10. Drug Analysis by SPME10.1.
Fundamentals of Extraction10.3. Fibre Selection: Adsorption Versus Absorption10.4. Considerations of Drug Properties10.5. Novel SPME Coatings for LC10.7. Instrumental Configurations10.9. Ligand—Receptor Binding and Determination of Free Concentrations11.1. Analysis of Biological Samples11.3.
Determination of Free Concentrations and Binding Constants11.4. Calibration of SPME for Bioanalytical Applications11.5. In Vivo Sampling with Solid-Phase Microextraction12.1. In Vivo Method Development12.3. In Vivo Applications12.4.
Solid-Phase Microextraction Protocols13.1. Protocol for Automated High-Throughput SPME-LC using the Concept 96 Robotic Sample Preparation Station13.2.
Protocol for Automation of Ligand-Receptor Binding Studies Using Concept 9613.3. In Vivo SPME Protocol for Direct Monitoring of Circulating Intravenous Blood Concentrations13.4. Protocol for Setting up Automated SPME-GC Methods. The primary focus of Professor Pawliszyn's research program is the design of highly automated and integrated instrumentation for the isolation of analytes from complex matrices and the subsequent separation, identification and determination of these species.
The primary separation tools used by his group are Gas Chromatography, Liquid Chromatography and Capillary Electrophoresis coupled to variety of detections systems, including range of mass spectrometry techniques. Currently his research is focusing on elimination of organic solvents from the sample preparation step to facilitate on-site monitoring and in-vivo analysis. Several alternative techniques to solvent extraction are investigated including use of coated fibers, packed needles, membranes and supercritical fluids.
Pawliszyn is exploring application of the computational and modeling techniques to enhance performance of sample preparation, chromatographic separations and detection. The major area of his interest involves the development and application of imaging detection techniques for microcolumn chromatography, capillary electrophoresis and micro chip separation devices.He is an author of over 400 scientific publications and a book on Solid Phase Microextraction. His Hirsch Index (H-index) is 69. He is a Fellow of Royal Society of Canada and Chemical Institute of Canada, editor of Analytica Chimica Acta, Trends in Analytical Chemistry and a member of the Editorial Board of Journal of Separation Science. He initiated a conference, 'ExTech', focusing on new advances in sample preparation and disseminates new scientific developments in the area, which meets every year in different part of the world.
He received the 1995 McBryde Medal, the 1996 Tswett Medal, the 1996 Hyphenated Techniques in Chromatography Award, the 1996 Caledon Award, the Jubilee Medal 1998 from the Chromatographic Society, U.K., the 2000 Maxxam Award from Canadian Society for Chemistry, the 2000 Varian Lecture Award from Carleton University, the Alumni Achievement Award for 2000 from Southern Illinois University, the Humboldt Research Award for 2001, 2002 COLACRO Medal, 2003 Canada Research Chair, in 2006 he has been elected to the most cited chemists by ISI, in 2008 he received A.A. Benedetti-Pichler Award from Eastern Analytical Symposium, 2008 Andrzej Waksmundzki Medal from Polish Academy of Sciences, 2008 Manning Principal Award, 2010 Torbern Bergman Medal from the Swedish Chemical Society, 2010 Ontario Premier's Innovation Award, 2010 Marcel Golay Award, 2010 ACS Award in Separation Science and Technology and 2011 PittCon Dal Nogare Award. He presently holds the Canada Research Chair and Natural Sciences and Engineering Research Council of Canada Industrial Research Chair in New Analytical Methods and Technologies. He presently holds the University Professor title, the Canada Research Chair and NSERC Industrial Research Chair in New Analytical Methods and Technologies. His Hirsh Index ('H' Index) is 70.
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